P-687 Serum Anti-Müllerian hormone to antral follicle count ratio does not predict euploidy rate in young patients who had at least 1 zygote after fertilization

Abstract Study question Does serum Anti-Müllerian hormone to antral follicle count ratio predict euploidy rate in young patients who had at least 1 zygote after fertilization? Summary answer Serum (AMH/AFC) does not fertilization. What is known already AMH produced by the granulosa cells of growing follicles. It has been demonstrated that follicles are main contributors its values. AMH´s role as a quantitative predictor ovarian response stimulation undeniable. However, studies looking correlation with oocyte quality, assessed embryo ploidy, have yielded conflicting results. All them include only one blastocyst for biopsy obviating those all embryos arrested, which might be genetically abnormal. Besides, no study before considered per-follicle production when evaluating levels. design, size, duration Retrospective analysis women undergoing Preimplantation Genetic Testing Aneuploidy stage according standard clinical procedures tertiary referral IVF center from April 2017 August 2022. As linear regression excluded an effect age on until 35 years, 570 below this were included. AMH/AFC was calculated marker production. Patients classified into quartiles. Euploidy rates/zygote compared among them. Participants/materials, setting, methods measured Elecsys within 6 months start stimulation. Scans performed 3 experienced sonographers. Women > 5,98 ng/ml, endometriosis, autoimmune disease, non-functional cyst, hormonal treatment prior measurement or history surgery, men less than million sperm/milliliter ejaculate, and couples abnormal karyotype gonadotoxic excluded. Main results chance Included median (+ interquartile range) 2.73 ng/ml (1.68-3.77), BMI 25.7 kg/m2 (22.8-28.9) 14 (11-19). Median number oocytes collected (10-20) metaphase II 11 (7-15). Thresholds p25th p75th ratios 0.13 0.24 respectively. The included <p25th, p25th-p75th >p75th groups 134, 297 139, Whereas wereńt statistically different groups, significant differences found (<p25th: 1.38 (0.8-2.1); p25th-p75th: 2.75 (2-3.7); >p75th: 3.9 (3.1-4.8), p < 0.001) AFC (9.2-20) 16 (12-20); 13 (10-16), 0.001). No fertilization 0.75 (0.6-0.8); (0.6-0.9); 0.78 (0.7-0.9)) nor rate/zygote 0.64 (0.5-0.8), 0.6 (0.4-0.8), (0.5-0.7)). wasńt groups: <p25th: 0.36 (0.2-0.5); 0.33 (0.2-0.5). When p25th-p75th, <p25th showed tendency towards higher risk having euploid embryo, due culture arrest aneuploidy (19.4% vs 10.8%, 0.065). Limitations, reasons caution retrospective design restrict adequate control confounding factors. small size lower quartile group compromise accuracy findings. Wider implications findings association between levels live birth In Vitro Fertilization remains debated. didńt find rate/zygote, further research will evaluate other markers quality affected low AMH. Trial registration Not applicable